hSERT Mutation | Kma | nb | COS7 Cell | Xenopus Oocytes | ||
---|---|---|---|---|---|---|
Vmaxa,c | nb | 5-HT Uptaked | ne | |||
µM | % of WT | % of WT | ||||
WT | 1.31 ± 0.16 | 15 | 100 | 15 | 100 | 31 |
C109A | 1.78 ± 0.16 | 15 | 93 ± 4 | 15 | 84 ± 6 | 21 |
C109A-Q111C | 1.32 ± 0.29 | 7 | 34 ± 3* | 7 | 9 ± 2* | 11 |
C109A-S190C | 0.82 ± 0.14 | 5 | 69 ± 5* | 5 | 81 ± 5 | 20 |
C109A-S224C | 1.36 ± 0.35 | 4 | 64 ± 7* | 4 | 5 ± 1* | 3 |
C109A-V236C | 1.08 ± 0.17 | 6 | 43 ± 2* | 6 | 14 ± 5* | 5 |
C109A-S252C | 1.37 ± 0.08 | 6 | 97 ± 5 | 6 | 33 ± 7* | 16 |
C109A-T301C | 1.42 ± 0.23 | 8 | 44 ± 4* | 8 | 11 ± 2* | 6 |
C109A-T323C | 1.56 ± 0.14 | 7 | 79 ± 10 | 4 | 52 ± 4* | 22 |
C109A-S395C | 1.44 ± 0.32 | 4 | 75 ± 15 | 4 | 41 ± 7* | 9 |
C109A-A401C | 1.45 ± 0.09 | 5 | 81 ± 9 | 5 | 14 ± 2* | 7 |
C109A-A419C | 1.74 ± 0.13 | 4 | 116 ± 17 | 4 | 111 ± 9 | 16 |
C109A-L481C | 1.38 ± 0.09 | 4 | 78 ± 4 | 4 | 82 ± 9 | 8 |
C109A-S574C | 1.93 ± 0.23 | 4 | 94 ± 9 | 4 | 27 ± 3* | 13 |
↵a Values are mean ± S.E.M.
↵b Number of individual uptake saturation experiments performed as described in the legend to Fig. 1 and in Materials and Methods.
↵c Expressed as percentage of WT determined in parallel. For WT, Vmax (mean ± S.E.M.) was 109 ± 12 fmol/min per well (n = 15).
↵d Values are mean ± S.E.M. of the uptake measured at a concentration of 50 nM [3H]5-HT and expressed as the percentage of uptake in WT hSERT-expressing oocytes assayed in parallel. For WT, the 5-HT uptake (mean ± S.E.M.) is 1.58 ± 0.11 pmol/min per oocyte (n = 37).
↵e Number of individual oocytes.
↵* P < 0.05, significantly different from WT (analysis of variance).