TABLE 1

Primers used for site-directed mutagenesis

PrimerSequenceAmino Acid Change
1fwd5′ CTCAAGTTGGAAACCACGGCGAAGCGTGATGAAGTAGAGG 3′S254A
1rev5′ CCTCTACTTCATCACTCTTCGCCGTGGTTTCCAACTTGAG 3′
2fwd5′ GCTCCTCAGACAGAGGCGAAGCAGATTTTGG 3′W278A
2rev5′ CCAAAATCTGCTTCGCCTCTGTCTGAGGAGC 3′
3fwd5′ AACCGATTTGCCAGCGGTGCGTAAGCGGAC 3′L447A
3rev5′ GTCCGCTTACGCACCGCTGGCAAATCGGTT 3′
  • fwd, forward; rev, reverse.