TABLE 2

Results of kinetic modeling

Results of kinetic modeling of single-channel currents elicited by 50 µM GABA alone or in the presence of e18,19dinor3α5α16O, 3α5α17βEt, or e3α5α4βOMe16O. Data from four to six patches at each condition were combined and analyzed using Model 1. The rate constants (and standard deviations estimated from the Hessian matrix) are in s⁻¹. The association (3 µM⁻¹s⁻¹) and dissociation rate constants (300 s⁻¹) for GABA, and the rate for A₂C₁→A₂C₃ transition (300 s⁻¹) were constrained to values determined in a previous analysis (Lema and Auerbach, 2006). Single-channel data were filtered at 2 kHz. The dead time was 90 µs. The rate constants in this table were used to simulate synaptic events (Fig. 4).