Pharmacological parameters of the results shown in Fig. 8, A–C
The results are the mean ± S.E.M. of least eight independent experiments, each in quadruplicate. Top and bottom are expressed as fold of basal, and log EC50 is expressed as logged EC50 (M). The statistical significance of the difference in values between the wild type (WT) and mutant was determined using a one-sided t test.
| WT | H17A/H19A | |||
|---|---|---|---|---|
| ZnCl2 (+) (N = 13) | ZnCl2 (−) (N = 13) | ZnCl2 (+) (N = 8) | ZnCl2 (−) (N = 8) | |
| GPR39-C3 | ||||
| Top | 7.68 ± 0.11 | N.D. | 6.01 ± 0.13* | N.D. |
| Bottom | 1.38 ± 0.17 | N.D. | 1.37 ± 0.18 | N.D. |
| Log EC50 | −6.72 ± 0.10 | N.D. | −6.49 ± 0.12* | N.D. |
| Hill slope | 1.01 ± 0.37 | N.D. | 1.22 ± 0.22 | N.D. |
| LY2784544 | ||||
| Top | 7.24 ± 0.20 | N.D. | 4.86 ± 0.13* | N.D. |
| Bottom | 1.20 ± 0.06 | N.D. | 1.17 ± 0.04 | N.D. |
| Log EC50 | −7.37 ± 0.05 | N.D. | −7.08 ± 0.05* | N.D. |
| Hill slope | 1.13 ± 0.12 | N.D. | 1.35 ± 0.17 | N.D. |
| GSK2636771 | ||||
| Top | 4.80 ± 0.12 | N.D. | 3.84 ± 0.12* | N.D. |
| Bottom | 1.10 ± 0.05 | N.D. | 1.06 ± 0.03 | N.D. |
| Log EC50 | −7.09 ± 0.05 | N.D. | −6.46 ± 0.06* | N.D. |
| Hill slope | 1.29 ± 0.17 | N.D. | 1.20 ± 0.16 | N.D. |
N.D., could not determine.
↵* p < 0.05.