Table 4

Effects of transferring the E loop on actions of other potentiators.

Subunits injectedPotentiatorRelative responseP Value a
Mean ± S.E.No. of CellsP to 1P to pairP to free
α4&β2 8:1GAL 0.50.94 ± 0.0370.09-nd
β-α&α(E)GAL 0.50.95 ± 0.0260.081.0nd
α4&β2 1:8GAL 0.51.01 ± 0.0490.85-nd
β-α&β(E)GAL 0.50.91 ± 0.0360.030.24nd
α4&β2 8:1GAL 100.52 ± 0.026<0.001-nd
β-α&α(E)GAL 100.88 ± 0.0360.01<0.001nd
α4&β2 1:8GAL 100.90 ± 0.0280.004-nd
β-α&β(E)GAL 100.68 ± 0.0650.0050.001nd
α4&β2 8:1ATR 1001.51 ± 0.058<0.001-nd
β-α&α(E)ATR 1000.29 ± 0.037<0.001<0.001nd
α4&β2 1:8ATR 1000.35 ± 0.067<0.001-nd
β-α&β(E)ATR 1000.55 ± 0.048<0.0010.002nd
α4&β2 8:1ZN 1001.97 ± 0.0511<0.001--
α-βZN 1002.98 ± 0.1211<0.001-<0.001
α-β&α(E)ZN 1002.85 ± 0.176<0.0011.0<0.001
β-α&αZN 1001.38 ± 0.0540.005-0.01
β-α&α(E)ZN 1001.50 ± 0.077<0.0011.00.02
α4&β2 1:8ZN 1000.21 ± 0.026<0.001--
α-β&β(E)ZN 10011.17 ± 0.846<0.001-<0.001
β-α&βZN 1000.37 ± 0.037<0.001-1.0
β-α&β(E)ZN 1003.59 ± 0.417<0.001<0.001<0.001
  • Three additional potentiating drugs were tested: galantamine at 0.5 μM (GAL 0.5) and 10 μM (GAL 10), atropine at 100 μM (ATR 100) and Zn2+ at 100 μM (ZN 100). Means ± S.E. and the number of cells are presented for the ratio of the response to a low concentration of ACh in the presence of physostigmine to the response of the same cell to ACh in the absence of physostigmine.

  • a Three P values are given: “to 1” indicates the P value for the difference to 1 (no effect) (one-sample t test), “to pair” indicates that the relative response differs from the value immediately above [e.g., α4&β2 1:8 to β-α&β(E)] (one-way analysis of variance on all subunit combinations in the group with Bonferroni post hoc correction), and “to free” gives the P value for the difference from the value at the top of the group (one-way analysis of variance on all conditions in the group with Bonferroni post hoc correction). The 5 groups consisted of the 4 subunit combinations tested with 0.5 μM galantamine, with 10 μM galantamine, and with 100 μM atropine, then the 5 subunit combinations starting with α4&β2 8:1 tested with 100 μM zinc and finally the 4 subunit combinations tested with 100 μM zinc starting with α4&β2 1:8.

    Dashes indicate that that particular subunit combination was the comparison value for the P computation and “nd” indicates that the comparison was not made.