Responses of mIPSCs to acute drug (EtOH) application in DGCs
τ1 and τ2 are time constants (mean ± S.E.M.) when the decay of mIPSCs was fitted with two exponentials. Decay of mIPSCs in pattern d was fitted well with only one exponential (MiniAnalysis program V6.07). α4KO, α4 subunit knockout mice; E0, without EtOH; E50, in the presence of 50 mM EtOH.
| Pattern a | Pattern b | Pattern c | Pattern d | ||||
|---|---|---|---|---|---|---|---|
| τ1 (ms) | τ2 (ms) | τ1 (ms) | τ2 (ms) | τ1 (ms) | τ2 (ms) | τ (ms) | |
| CIV E0 | 7.8 ± 0.4 | 32.0 ± 11.1 | 14.1 ± 1.2 | 54.9 ± 11.3 | 16.9 ± 0.6 | ||
| E50 | 8.0 ± 0.4 | 60.3 ± 11.6 | 16.3 ± 0.7* | 58.4 ± 14.6 | 18.2 ± 0.5 | ||
| CIE E0 | 4.0 ± 0.3 | 12.9 ± 2.2 | 14.6 ± 0.7 | 70.4 ± 9.2 | 17.4 ± 0.7 | ||
| E50 | 8.0 ± 0.3* | 26.9 ± 4.3* | 17.2 ± 0.3* | 73.1 ± 12.5 | 19.5 ± 0.4* | ||
| WT E0 | 7.6 ± 0.5 | 37.1 ± 10.5 | 13.7 ± 0.6 | 35.9 ± 8.9 | 17.0 ± 0.5 | ||
| E50 | 7.7 ± 0.4 | 53.9 ± 3.6 | 14.8 ± 0.5 | 50.7 ± 13.5 | 18.0 ± 0.5 | ||
| α4KO E0 | 7.1 ± 0.4 | 28.0 ± 8.6 | 13.8 ± 0.9 | 35.4 ± 8.6 | 16.6 ± 1.1 | ||
| E50 | 8.2 ± 0.5* | 48.0 ± 12.4 | 16.2 ± 1.1* | 63.7 ± 19.9 | 18.4 ± 0.8* | ||
↵* P < 0.05, the decay time constant of a pattern in the presence of 50 mM EtOH is statistically different from that of 0 EtOH (two-way repeated measures ANOVA, followed by Sidak method for pairwise multiple comparison). n = 6 neurons from three rats/group. n = 5 neurons from two mice/group.