Time course of macroscopic NMDA receptor responses to glycine and AICP
Data are from whole-cell patch-clamp recordings of rat GluN1/2B and GluN1/2C receptors expressed in HEK-293 cells. Here, 100 µM glycine or 10 µM AICP were applied using rapid solution exchange in the continuous presence of 100 µM glutamate (see Materials and Methods). Steady-state (SS) and peak responses were normalized to the glycine response from the same cell. The deactivation time course was described using mono-exponential fits, and the time constant is listed (τdeactivation). N is the number of cells, and the values are mean ± S.E.M.
| Response | GluN1/2B | GluN1/2C | ||
|---|---|---|---|---|
| Glycine | AICP | Glycine | AICP | |
| Rise time (10%–90%, ms) | 16 ± 2 | 74 ± 12 | 6.2 ± 0.6 | 46 ± 2 |
| SS/peak amplitude | 89 ± 1 | 81 ± 3 | 100 ± 1 | 97 ± 1 |
| Peak amplitude (% of glycine) | 100 | 8.1 ± 1.1 | 100 | 388 ± 18 |
| SS amplitude (% of glycine) | 100 | 7.4 ± 1.0 | 100 | 376 ± 18 |
| τdeactivatiom (s) | 0.820 ± 0.033 | 22.6 ± 4.2 | 0.662 ± 0.021 | 84.3 ± 22.0 |
| N | 8 | 8 | 5 | 5 |