TABLE 2

Binding affinities or potencies of PYY-(3-36) for WT-hY2 and mutants

Y2 and mutantsPYY-(3-36)Fold change (WT/mut)n
Ki or EC50 (nM)a,b95% CIc
WT-hY20.380.31; 0.44143
0.31a0.25; 0.3815
Cys1032.57Phea73**18; 2832353
Tyr1102.64Ala1.4*0.33; 5.63.63
Thr1112.65Ala12**4.2; 37333
Thr1112.65Leu0.540.095; 3.11.43
Pro1273.29Alaa8.0**0.35; 182263
Pro1273.29Leua142**43; 4664653
Arg1874.64Ala1.5**0.92; 2.64.17
Glu1884.65Ala0.130.07; 0.290.34
Glu2055.24Leu8.5**2.8; 26233
Ser2205.39Ala2.2**0.60; 8.35.93
Ser2235.42Ala0.550.15; 2.01.53
Ser2235.42Leu0.390.06; 2.61.03
Leu2846.51Tyr0.160.09; 0.290.43
His2856.52Phe0.100.03; 0.380.34
Gln2886.55Ala0.730.13; 4.31.93
Gln2886.55Leu1.3**0.79; 2.23.57
Gln2886.55Glu1.00.15; 7.42.73
Gln2886.55Arg30**18; 49797
Tyr3037.31Ala0.770.40; 1.523
Phe3077.35Ala17**8.4; 34455
  • a Affinity (Ki) or potency (EC50, in italics) values are calculated as the geometric mean of the n independent experiments.

  • b One-way ANOVA with Dunnett post hoc analysis were used for pKi, (or potency in pEC50, respectively). Asterisks indicate the statistical significance of differences of PYY-(3-36) affinity in pKi, (or potency in pEC50, respectively) between Y2 and its mutants (*P < 0.01; **P < 0.001).

  • c 95% confidence interval (CI), given as [lower; upper].