TABLE 3

Apparent K_m values of MPP⁺ and TEA⁺ transport mediated by rOCT1 wild-type and rOCT1 mutants

Initial uptake rates of nine different concentrations radioactively traced MPP⁺ or TEA⁺ were measured at 37°C in stably transfected HEK293 cells and apparent K_m values were determined by fitting the Hill equation to data of individual experiments. Mean values ± S.D. of three or four separate experiments are shown. The compiled uptake measurements and the calculated Hill coefficients are shown in Figs. 3 and 4.