Arrestin3-Rluc Recruitment, 1 µM NPY | 10 µM NPY | |||
---|---|---|---|---|
BRET50 | netBRET | Kobs/min−1 | Kobs/min−1 | |
WT Y2R | 0.0011 (0.0005–0.0017) | 0.429 (0.384–0.474) | 0.286 (0.210–0.362) | 0.292 (0.234–0.351) |
W6.48Y | 0.0020 (0–0.0045) | 0.241 (0.137–0.345)*** | 0.618 (0.391–0.845) | 0.518 (0.232–0.805) |
W6.48H | 0.0044 (0.0004–0.0084) | 0.176 (0.100–0.251)*** | 0.357 (0.233–0.480) | 0.323 (0.219–0.427) |
W6.48T | 0.0022 (0.007–0.0037) | 0.222 (0.161–0.283)*** | 0.452 (0.258–0.645) | 0.391 (0.271–0.511) |
↵*** P < 0.001.