TABLE 3

Functional affinity (pKB) and efficacy (logτB) parameters for NPS R568 and AC265347 determined in Ca2+i mobilization assays in the absence of ambient divalent cations

Data are mean ± S.E.M. and 95% confidence intervals from the indicated number of independent experiments (n).

NPS R568AC265347
pKB (95% CI)LogτB (95% CI) (τB)npKB (95% CI)LogτB (95% CI) (τB)n
WTNDND85.43 ± 0.10a (5.23–5.63)0.05 ± 0.10 (0.01–0.11) (1.1)8
G670ECL1A5.71 ± 0.18a (5.28–6.13)−0.19 ± 0.04 (−0.27 to −0.11) (0.6)76.13 ± 0.08b (5.96–6.31)0.07 ± 0.03 (0.04–0.11) (1.2)7
E8377.32DNDND85.90 ± 0.14a,b (5.60–6.19)−0.03 ± 0.03 (−0.10 to 0.03) (0.9)8
A8407.35VNDND7NDND7
A8447.39V6.48 ± 0.32 (5.78–7.21)−0.27 ± 0.06 (−0.42 to −0.17) (0.5)66.89 ± 0.20b (6.51–7.31)−0.16 ± 0.04b (−0.25 to −0.09) (0.7)6
  • CI, confidence interval; ND, not determined due to negligible efficacy and/or low affinity.

  • a Significantly lower than in the presence of ambient divalent cations (P < 0.05, two-way ANOVA with Sidak’s comparisons post-test).

  • b Significantly different from WT (P < 0.05, one-way ANOVA with Dunnett’s multiple comparisons post-test).