Inhibition of CD45 reduces 5C3 ascites growth and mAb production in vivo
5C3 hybridoma cells, which are syngeneic to Balbc/C mice, were used in tumor studies. On day 0, 1 × 106 5C3 tumor cells were injected intraperitoneally, and they grew and generated ascitic fluid. At day 4 mice (n = 8 per group) received intraperitoneal injections of either 3 mg/kg compound 211 or vehicle at days 4, 6, 8, and 10. At day 11 all peritoneal fluid was collected; the cell numbers were counted and mAb titers quantified by serial dilution (a high dilution indicates a high titer of mAb). Average ± S.D., n = 8.