Primer sequences for single-cell reverse-transcription polymerase chain reaction
| Gene | Primer | Sequence (5′ to 3′) | Product Size (bp) |
|---|---|---|---|
| β-Actin | Forward | GTGGGAATGGGTCAGAAGG | 302 |
| Reverse | GAGGCATACAGGGACAGCA | ||
| MRGPRA3 | Forward | CGACAATGACACCCACAACAA | 150 |
| Reverse | GGAAGCCAAGGAGCCAGAAC | ||
| TRPV1 | Forward | GCTGCTAACGGGGACTTCTT | 285 |
| Reverse | CTTCAGTGTGGGGTGGAGTT | ||
| NaV1.1 | Forward | AGACAGCATCAGGAGGAAGG | 118 |
| Reverse | GGAGAACAGGGAACCACGA | ||
| NaV1.2 | Forward | CATTTTCGGCTCATTCTTCA | 305 |
| Reverse | CATCTCTTGGCTCTGGTCGT | ||
| NaV1.3 | Forward | AGACAGAGGGAGCACTTGGA | 200 |
| Reverse | CTATTGCGTCTTGGGGAAAA | ||
| NaV1.6 | Forward | AGGCAGCAAAGACAAACTGG | 157 |
| Reverse | GCAGCACTTGAACCTCTGG | ||
| NaV1.7 | Forward | ATGCTCTTCTTTGCGGTTTC | 381 |
| Reverse | CCTGCTCTTTTCGGCTTCTT | ||
| NaV1.8 | Forward | CAATCCGACCCTTACAACCA | 147 |
| Reverse | AAAGACCCCGTCATCCAAG | ||
| NaV1.9 | Forward | CAGCTTTGGCTGGTCTTTTC | 228 |
| Reverse | TTCTCCTTGGCCTCTGTCTC |