WT | N410K+T423K | |||||
---|---|---|---|---|---|---|
pKBa | LogαβAChb | nc | pKBa | LogαβAChb | n | |
Gallamine | 6.08 ± 0.16 | −3# | 3 | 4.12 ± 0.21* | −3# | 4 |
Strychnine | 5.13 ± 0.05 | −3# | 3 | 3.55 ± 0.36* | −3# | 4 |
Brucine | 5.42 ± 0.14 | −3# | 3 | 4.21 ± 0.22* | −3# | 4 |
↵a Negative logarithm of the allosteric modulator equilibrium dissociation constant.
↵b Logarithm of the functional cooperativity for the interaction between the modulator and ACh; in all instances, the interaction was indistinguishable from competition (F-test), indicative of very high negative cooperativity (αβ → 0), and thus cooperativity values were constrained to an arbitrarily low value, #.
↵c Number of experiments performed.
↵* Significantly different from the corresponding value at the wild type (P < 0.05), Student’s t test.
↵# Arbitrarily low value.