TABLE 1

Small-angle X-ray scattering data collection and scattering-derived parameters

SampleWTD188Y
Data collection parameters
 BeamlineESRF BM29ESRF BM29
 Beam geometry (mm2)0.7 × 0.70.7 × 0.7
 Wavelength (Å)1.01.0
 Q range (Å−1)0.0025–0.50.0025–0.5
 Exposure per frame (s)a0.50.5
 Concentrations (mg/ml)8.7, 4.3, 2.1, 0.610.0, 5.0, 2.5, 1.1
 Temperature (°C)55
Structural parameters
 Rg (Å) [from P(r)]b39.9 ± 0.0139.7 ± 0.01
 Rg (Å) (from Guinier)b39.3 ± 0.139.6 ± 0.1
 Dmax (Å)c108 ± 10.8109 ± 10.9
 Porod volume [from P(r)] (103 Å3)316.15312.0
 Estimated mass (kDa)c186.0 ± 9.2183.5 ± 5.5
Software employed
 Primary data reductionAUTOMARAUTOMAR
 Data processingPRIMUS, GNOMPRIMUS, GNOM
  • Dmax, maximal intramolecular distance; ESRF, European Synchrotron Radiation Facility; Rg, radius of gyration.

  • a Ten frames were measured for each sample.

  • b ±S.E.

  • c ±10% (estimated range).