TABLE 1

Level (%) of 1 mM Zn²⁺ inhibition of 100 µM ACh currents at mouse α3β4 nAChRs under different chronic (24–30 h) treatments

Cells were studied 52.5–52.75 h after the start of transfection and 26.5–27.25 h after the start of incubation in no drug-containing media or after addition of menthol and/or nicotine (both durations are averages). Mean ± S.E. values are represented in the table.

Chronic Treatment	Control	(−)-Menthol	Nicotine	Nicotine and (−)-Menthol
(−)-Menthol (nM)	0	500	0	500
Nicotine (µM)	0	0	250	250
% Zn²⁺ inhibition^{^a} (%)	50.1 ± 6.5	48.5 ± 4.8	48.7 ± 6.1	55.4 ± 5.3
N	14	16	19	20
Cell capacitance (pF)^{^a}	28.2 ± 5.3	23.3 ± 2.5	22.5 ± 1.9	22.9 ± 1.9
Current density (pA/pF)^{^a}^,^{^b}	−108.4 ± 31.6	−91.4 ± 15.6	−62.7 ± 10.4	−71.9 ± 12.3

↵^a P > 0.05 for one-way ANOVA with post-hoc Tukey HSD test.
↵^b Current density between these same chronic treatments with higher N numbers for control and chronic (−)-menthol treatment are shown in Table 3 and discussed in Results in the section entitled Chronic Nicotine But Not Chronic Menthol Treatment Reduces Functional PM Mouse α3β4 nAChR Levels.