Mutant | EC50 Value | |||
---|---|---|---|---|
5-HTa | mCPGBa | |||
TEVC | ∆F | TEVC | ∆F | |
µM | µM | µM | µM | |
WT | 2.6 [2.2; 3.0] | N.D. | 0.45 [0.35; 0.60] | N.D. |
WT + TAMRA | 3.5 [3.1; 4.7]* | N.D. | 0.95 [0.70; 1.3] | N.D. |
Y89C | 0.24 [0.20; 0.28]* | N.D. | 0.48 [0.34; 0.68] | N.D. |
Y89C + TAMRA | 0.70 [0.56; 0.86]* | 4.0 [3.2; 4.9]# | 0.26 [0.20; 0.34] | 1.0 [0.67; 1.5] |
Q146C | 1.2 [0.9; 1.7]* | N.D. | 1.5 [1.4; 2.0] | N.D. |
Q146C + TAMRA | 4.8 [3.0; 7.9] | 61 [48; 79]# | 1.1 [0.90; 1.45] | 49 [36; 65]# |
F221C | 85 [76; 94]* | N.D. | N.D. | N.D. |
F221C + TAMRA | >300* | >300 | N.D. | N.D. |
M223C | 7.2 [5.2; 10]* | N.D. | 3.7 [ 3.0; 4.5]* | N.D. |
M223C + TAMRA | 22 [19; 26]* | 18 [14; 23] | 8.9 [6.1; 13]* | 24 [15; 38] |
TEVC, two-electrode voltage clamp. N.D., not determined.
↵a The EC50 values were determined as described in Materials and Methods by nonlinear fitting of concentration-inhibition data collected at 5–10 oocytes. Numbers in brackets denote the 95% confidence interval for EC50.
↵* P < 0.01 vs. WT. #P < 0.01 vs. two-electrode voltage-clamp EC50 (ANOVA with Dunnet’s correction for multiple comparisons).