EC50 values for fluorescence responses to antagonists at mutant h5-HT3A receptors
| Reporter Mutant | EC50 Valuea | |||
|---|---|---|---|---|
| Ondansetron | Granisetron | Encenicline | Tropisetron | |
| nM | nM | nM | nM | |
| Y89C | 58 [36, 96] | 100 [59; 180] | 170 [110; 270] | 45 [24; 87] |
| Q146C | 850 [73; 1000] | 800 [670; 970] | 370 [340; 410] | 850 [540; 1300] |
| M223C | 51 [38; 70] | 54 [38; 77] | 74 [59; 95] | 38 [19; 74] |
↵a The EC50 values were determined as described in Materials and Methods by nonlinear fitting of concentration-inhibition data collected at 5–10 oocytes. Numbers in brackets denote the 95% confidence interval for EC50.