Simulation of agonist concentration-response relationships upon changes in binding or transducer slopes and τA
Data were simulated using the cooperative agonist operational model (eq. 2) and a Hill equation (eq. 9) was fitted to simulated data to determine agonist potency, maximum effect, and Hill slope.
| Parameter | eq. 2 | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| nB 0.5 | nB 1 | nB 2 | nB 3 | |||||||||
| pEC50 | Emax | nH | pEC50 | Emax | nH | pEC50 | Emax | nH | pEC50 | Emax | nH | |
| τA 1 | ||||||||||||
| nT 0.5 | 4.8 | 51 | 0.3 | 3.9 | 50 | 0.7 | 3.4 | 50 | 1.3 | 3.3 | 50 | 2.0 |
| nT 1 | 3.5 | 51 | 0.5 | 3.2 | 51 | 1.0 | 3.1 | 51 | 2.0 | 3.0 | 51 | 3.0 |
| nT 2 | 2.6 | 51 | 0.6 | 2.8 | 51 | 1.3 | 2.8 | 51 | 2.4 | 2.9 | 51 | 3.5 |
| nT 3 | 2.2 | 52 | 0.7 | 2.6 | 52 | 1.4 | 2.7 | 52 | 2.9 | 2.8 | 52 | 4.1 |
| τA 3 | ||||||||||||
| nT 0.5 | 5.2 | 66 | 0.3 | 4.1 | 65 | 0.6 | 3.5 | 64 | 1.3 | 3.3 | 64 | 1.9 |
| nT 1 | 4.2 | 76 | 0.5 | 3.5 | 76 | 1.0 | 3.2 | 76 | 2.0 | 3.1 | 76 | 3.0 |
| nT 2 | 3.7 | 90 | 0.8 | 3.3 | 90 | 1.5 | 3.1 | 91 | 3.3 | 3.1 | 91 | 4.9 |
| nT 3 | 3.6 | 96 | 1.1 | 3.3 | 97 | 2.1 | 3.1 | 97 | 4.7 | 3.0 | 97 | 6.0 |
pEC50, agonist potency.