Parameter | ACh vs. LY2033298 (eqs. 3 and 4)^{a} |
---|---|

pK_{A} [K_{A} (µM)] | 6.0 (1.0)^{b} |

pK_{B} [K_{B} (µM)] | 5.9 ± 0.3 (1.3) |

Log τ_{A} (τ_{A}) | 0.9 ± 0.1 (7.9) |

Log τ_{B} (τ_{B}) | 0.5 ± 0.2 (3.2) |

Log αβ (αβ) | 0.7 ± 0.3 (5.0) |

n | 1.0^{c} |

E_{m} (% ACh maximum) | 112 ± 5.0 |

Dfd, degrees of freedom denominator; Dfn, degrees of freedom numerator;

*E*_{m}, maximum system response; Log*αβ*(*αβ*), cooperativity; Log*τ*_{A}(*τ*_{A}), agonist efficacy; Log*τ*_{B}(*τ*_{A}), modulator efficacy;*n*, transducer or binding slope; p*K*_{A}(*K*_{A}), agonist affinity; p*K*_{B}(*K*_{B}), modulator affinity.↵

^{a}Data analyzed are from Leach et al. (2010).↵

^{b}The p*K*_{A}value was fixed to that determined in radioligand binding assays (Leach et al., 2010).↵

^{c}An*F*test determined that data were fitted best when the transducer or binding slopes were not different from unity. The*F*data used to test the hypothesis that*n*differed from 1 were the following: eq. 3*P*= 0.5766,*F*[(Dfn, Dfd) 0.3129 (1, 172)]; and eq. 4*P*= 0.4541,*F*[(Dfn, Dfd) 0.5629 (1, 172)].