EC50 (5-HT) and IC50 values (bupropion and hydroxybupropion) determined in X. laevis oocytes expressing 5-HT3ARs and 5-HT3ABRs.
Data represented as mean ± S.D. of n experiments. Statistical significance of A as compared with AB was determined with unpaired t test (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001). pEC50 and pIC50 are the negative logarithms of EC50 and IC50, respectively.
| Agonist | pEC50 (μM, Mean ± S.D.) | EC50 | nH (Mean ± S.D.) | n | |
|---|---|---|---|---|---|
| 5-HT3AR | 5-HT | 6.10 ± 0.02*** | 0.80 | 2.53 ± 0.58** | 5 |
| 5-HT3ABR | 5.38 ± 0.03*** | 4.30 | 1.04 ± 0.02** | 4 | |
| Antagonist | pIC50 (μM, mean ± S.D.) | IC50 | nH (Mean ± S.D.) | n | |
| 5-HT3AR | Bupropion | 4.06 ± 0.05*** | 87.1 | 1.28 ± 0.15*** | 5 |
| 5-HT3ABR | 3.09 ± 0.11*** | 840 | 1.78 ± 0.15*** | 7 | |
| 5-HT3AR | Hydroxybupropion | 3.95 ± 0.10*** | 113 | 1.17 ± 0.14*** | 5 |
| 5-HT3ABR | 3.28 ± 0.02*** | 526 | 1.80 ± 0.16*** | 8 |