TABLE 3

Main phase transition temperature (Tm) and cooperativity (B) of DMPC LUVs as a function of embedded fluorescent probe, medium pH, and diclofenac concentration

The displayed values are the mean ± S.D. of at least two independent assays. The membrane concentration of diclofenac ([Drug]m), calculated from eq. 5, is also presented.

pH[Drug] (×10−4 M)[Drug]m (×10−2 M)TMA-DPHDPH
Tm/°CBTm/°CB
3.00025.3 ± 0.5265 ± 4326.0 ± 0.1288 ± 5
41023.4 ± 0.191 ± 124.7 ± 0.1104 ± 7
82023.9 ± 0.180 ± 724.9 ± 0.871 ± 3
5.00024.7 ± 0.1295 ± 3424.7 ± 0.1317 ± 39
4823.6 ± 0.3132 ± 823.7 ± 0.2147 ± 16
81623.1 ± 0.386 ± 223.2 ± 0.191 ± 5
7.40024.7 ± 0.1358 ± 1524.9 ± 0.1389 ± 16
4624.5 ± 0.1284 ± 3424.8 ± 0.1321 ± 32
81224.1 ± 0.1223 ± 2224.5 ± 0.1273 ± 17