Compound | EC50 (µM) | Imax (% ACh Response) | n |
---|---|---|---|
Nic (N) | 0.430 ± 0.33 | 93.8 ± 14 | 11 |
1′-Et-NorN | 3.65 ± 2.1*** | 26.8 ± 10*** | 6 |
1′-MeN | 1.38 ± 0.45*** | 22.5 ± 5.4*** | 5 |
2′-MeN | 0.738 ± 0.089 | 83.4 ± 14 | 5 |
3′-Trans-MeN | 0.74 ± 0.42 | 52.9 ± 14*** | 5 |
3′-Cis-MeN | 3.83 ± 1.2***,ŧŧŧ | 51.4 ± 5.4*** | 5 |
4′-Trans-MeN | 0.254 ± 0.04 | 55.7 ± 27*** | 5 |
4′-Cis-MeN | 0.285 ± 0.14 | 32.7 ± 14*** | 4 |
5′-Trans-MeN | >300*** | <1*** | 4 |
5′-Cis-MeN | >300*** | <1*** | 4 |
n = the number of oocytes used.
* P < 0.05, **P < 0.01, ***P < 0.001, comparison with nicotine. (These P values were obtained from one-way ANOVA of log-transformed data with Dunnett’s multiple comparison post-test.)
ŧP < 0.05, ŧŧP < 0.01, ŧŧŧP < 0.001, comparison of cis- and trans-enantiomers; these P values were obtained from a two-tailed t test and, thus, are not corrected for multiple comparisons. Et, ethyl; Me, methyl; N, nicotine; NorN, nornicotine.