TABLE 2

Quantification of the UPR in live MCF-7:5C cells through measuring ThT relative intensity/cell

(A) ThT relative intensity/cell (mean and S.D.) with test compounds after 48-hour treatments. (B) ThT relative intensity/cell (mean and S.D.s) after 72-hour treatments. This reflects the differential capacity of test compounds in inducing EnR stress over time. ThT relative intensity/cell per treatment is representative of three biologic repeats.

(A) Day 2
CompoundRelative Intensity/Cell (Mean)S.D.
Veh0.2760.052
Thapsigargin0.8750.061
E41.2450.073
E20.7410.097
BMI-1350.4970.047
(B) Day 3
CompoundRelative Intensity/Cell (Mean)S.D.
Veh0.2960.057
Thapsigargin10.0550.068
BMI-1354.8780.049