List of phosphoproteomics studies of PGE2 signaling
The table gives an overview of the studies discussed in this review article. In addition to the four phosphoproteomics studies of PGE2 signaling in T cell, two related articles have also been included. Abbreviations: PMID - PubMed IDentifier, Ti4+ - titanium (IV) ion, IMAC - immobilized metal affinity chromatography.
| Study | Cell Type | Labeling | Enrichment | Data and Pathway Analysis Approaches | Notes |
|---|---|---|---|---|---|
| Oberprieler et al. (2010). High-resolution mapping of prostaglandin E2–dependent signaling networks identifies a constitutively active PKA signaling node in CD8+CD45RO+ T cells. PMID: 20558615 | Primary CD3+ | Stable isotope dimethyl labeling | PhosphositePlus, NetworKIN, Scansite, Phosida, IceLogo | Combined with phosphoflow | |
| Giansanti et al. (2013). Interrogating cAMP-dependent kinase signaling in Jurkat T cells via a protein kinase A targeted immune-precipitation phosphoproteomics approach. PMID: 23882029 | Jurkat | Stable isotope dimethyl labeling | PKA motif antibody enrichment | Ingenuity Pathway Analysis, Cytoscape, STRING, PhosphoSitePlus | |
| de Graaf et al., 2014. Single-step enrichment by Ti4+-IMAC and label-free quantitation enables in-depth monitoring of phosphorylation dynamics with high reproducibility and temporal Resolution. PMID: 24850871 | Jurkat | Label-free | STRING, NetworKIN, IceLogo, Gene Ontology analysis | ||
| Lone et al., 2021. Systems approach reveals distinct, joint signaling networks of the four PGE2 receptors in T Cells | Primary CD4+, CD8+, Tregs | Label-free | NetworKIN, IceLogo, Phonemes, STRING, PhosphoSitePlus, Predict Functional Phosphosites | Combined with phosphoflow Individual stimulation of 4 PGE2 receptors | |
| Gerarduzzi et al. (2014). Quantitative phosphoproteomic analysis of signaling downstream of the prostaglandin E2/G-protein coupled receptor in human synovial fibroblasts: potential antifibrotic networks. PMID: 25223752 | Primary synovial fibroblasts | Label-free | PKA motif antibody enrichment | Ingenuity Pathway Analysis | Related study |
| Beltejar et al. (2017). Analyses of PDE-regulated phosphoproteomes reveal unique and specific cAMP-signaling modules in T cells. PMID: 28634298 | Jurkat | Label-free | NetPhorest, Predict Functional Phosphosites, STRING, Gene Ontology Analysis | Related study. Combined stimulation with PDE inhibitors and PGE2 |