TABLE 2

Continuation of list from Table 1 of protein phosphosites in MA-10 cells that are increased by a combination of PDE4 and PDE8 inhibitors

Fold Incr.Protein Name/FunctionGene SymbolP04 sequence
315.7γ-Taxilin–ATF4/Creb2-binding proteinTxlng_KHS(ph)LEGDEGSDFITK_
325.6Serine/threonine-protein kinase SIK3Sik3/QSK_RFS(ph)DGAASIQAFK_
335.4Neuron navigator 1Nav1_KTS(ph)LDVSNSVEPGFLA
345.4cAMP-regulated phosphoprotein 19Arpp19DHIPTPQDLPQRKPS(ph)L
355.1Autophagy-related protein 16-1Atg16l1_RLS(ph)QPAGGLLDSITNI
365.1Protein ETHE1, mitochondrialEthe1_RLS(ph)QQSASGAPVLLR
374.9Perilipin-1Plin1_RLS(ph)TQFTAANELACR
384.7ADP-ribosylation factor GTPase-activating prot 1Arfgap1_RSS(ph)DSWDVWGSGSA
394.7SLAIN motif–containing protein 2Slain2_LS(ph)LQGHPTDLQTSNV
404.7TBC1 domain family member 10BTbc1d10b_RAS(ph)AGPVPGAVVIAE
414.6A-kinase anchor prot 1, mitochondrial, Dakap1Akap1_RLS(ph)EEACPGVLSVAPT
424.5RNA polymerase II nuclear localization proteinSlc7a6os_KTS(ph)DPDVILCNSVELIR
434.4TBC1 domain family member 25Tbc1d25;_RSS(ph)LTTAALPFTQSILS
444.4Vesicle-trafficking protein SEC22bSec22b_NLGS(ph)INTELQDVQR_
454.2Low-affinity cationic amino acid transporter 2Slc7a2_NLS(ph)LPFILHEK_
464.1Golgin subfamily A member 5Golga5_KS(ph)EPDDELLFDFLNSS
474.0CAP-Gly domain–containing linker protein 2Clip2_RYS(ph)LIDPASPPELLK_
484.0Nuclear factor related to κ-B–binding proteinNfrkb/INO80_KGS(ph)LAALYDLAVLKK_
493.9Pleckstrin homol domain family F m 2 (Phafin2)Plekhf2_RIS(ph)IVESCFGAAGQPL
503.9Cytoskeleton-associated protein 5Ckap5_KYS(ph)DTDIEPFLK_
513.8Cytospin-ASpecc1l_KGS(ph)SGNASEVSVACL
523.8Kinesin-associated protein 3Kifap3/Kap3_LSEVEQLLYYLQNRRDS(p
533.8GRB14 adapter proteinGrb14_RVT(ph)LPAITPIVLQK_
543.7Rho guanine nucleotide exchange factor 2Arhgef2_S(ph)LPAGDALYLSFNP
553.7Phospholipase DDHD2Ddhd2_KNS(ph)VSINRPAM(ox)S
563.5Protein FAM54BFam54b_AS(ph)FETLPNISDLCLK_
573.5Rho family GTPase activator (Alsin)Als2_RLS(ph)LPGLLSQVSPR_
583.5Reg of microtubule dynamics prot 3 (PTPIP51)Fam82a2_SHS(ph)LPNSLDYAQASE
593.5Serine/arginine repetitive matrix protein 2Srrm2_RSS(ph)SELSPEVVEK_
603.4Low-density lipoprot receptor adapter protein 1Ldlrap1/ARH_NQEGGDVPGTRRDS(ph)
  • Sites are ranked 31–60 based on fold response to the PDE inhibitor treatment. The underlined gene symbols in bold type face represent some of the phosphoproteins shown in Fig. 3 and 4.