List of protein phosphosites in MA-10 cells that are increased by a combination of the PDE4 inhibitor rolipram and PDE8 inhibitor PF-04957325
| Fold Incr. | Protein Name/Function | Gene Symbol | P04 sequence | |
|---|---|---|---|---|
| 1 | 18.7 | CAP-Gly domain–containing linker protein 1/2; | Clip1/2/Restin | _KIS(ph)GTTALQEALK_ |
| 2 | 12.8 | Regulator of microtubule dynamics protein 2 | Fam82a1 | _KFGS(ph)LTLPEESHSAQ |
| 3 | 12.3 | Rho guanine nucleotide exchange factor 11 | Arhgef11 | _KVS(ph)LLPGGGVGAAK_ |
| 4 | 12.2 | Transcoblamin uptake receptor (CD320 antigen) | Cd320 | _ESLLLSERKTS(ph)LI_ |
| 5 | 12.2 | DNA-binding protein A | Csda | _RRRS(ph)RPLNAVSQDGK |
| 6 | 12.1 | Acyl-CoA–binding domain–containing protein 5 | Acbd5 | _GERWGS(ph)RGSLNEQIA |
| 7 | 11.2 | Phosphatidylinositol 4-kinase β | Pi4kb | _RRLS(ph)EQLAHTPTAFK_ |
| 8 | 11.0 | Interferon regulatory factor 2–binding protein–like | Irf2bp2/Irf2bpl | _RQS(ph)CYLCDLPR_ |
| 9 | 9.8 | Nesprin-2 (Nuc envel spectrin-repeat proteins) | Syne2 | _RRES(ph)EEPTSPQSLCHL |
| 10 | 9.8 | Bcl-2–related ovarian killer protein | Bok | _RS(ph)SVFAAEIMDAFDR |
| 11 | 9.8 | RAF proto-oncogene Ser/Thr-protein kinase | Raf1 | -IVQQFGYQRRAS(ph)DDG |
| 12 | 9.0 | Carbamyl phosphate synthetase 2 | Cad | _RLS(ph)SFVTK_ |
| 13 | 9.0 | Bcl-2–related ovarian killer protein | Bok | _RSS(ph)VFAAEIMDAFDR |
| 14 | 8.9 | Serine/threonine-protein kinase WNK1 | Wnk1 | _KFS(ph)APGQLCVPMTSN |
| 15 | 8.2 | Cytoplasmic protein NCK1 | Nck1 | _RKPS(ph)VPDTASPADDS |
| 16 | 7.9 | 182-kDa tankyrase-1–binding protein | Tnks1bp1 | _RFS(ph)EGVLQPPSQDQE |
| 17 | 7.9 | Inositol 1,4,5-trisphosphate receptor type 3 | Itpr3 | _KQS(ph)VFGASSLPAGVG |
| 18 | 7.7 | Ras-spec guanine nuc-release factor RalGPS2 | Ralgps2 | _KS(ph)SAAAAAAAAAEGA |
| 19 | 7.4 | Heat shock protein HSP 90-β | Hsp90ab1 | _RLS(ph)ELLR_ |
| 20 | 7.3 | Zinc finger FYVE domain–containing protein 16 | Endofin | _RCS(ph)KPVCDLISDMGN |
| 21 | 7.3 | Protein G-protein–coupled receptor 107 | Gpr107 | _KVS(ph)NGAVEPQGSWE |
| 22 | 7.1 | Horm-sensitive lipase/Cholest ester hydrolase | Lipe | _RSS(ph)QGVLHMPLYTSPI |
| 23 | 6.8 | Phos b kinase reg subunit α, liver isoform | Phka2 | _GHRKS(ph)LNLVDSPQPL |
| 24 | 6.8 | Endoribonuclease Dicer | Dicer1 | _KIS(ph)LSPFSASDSAYEW |
| 25 | 6.7 | Ras-specific guan nuc-release factor RalGPS2 | Ralgps2 | _KSS(ph)AAAAAAAAAEGA |
| 26 | 6.5 | SH3 and PX domain-containing protein 2A | Sh3pxd2a | _RGS(ph)ADIIPLTATTPPCV |
| 27 | 6.2 | Carbohydrate-responsive element–binding protein | ChREBP | _RLS(ph)GDLNSIQPSGALS |
| 28 | 5.8 | Oxysterol-binding protein–related protein 11 | Osbpl11 | _RPS(ph)QNAMSFFNVGH |
| 29 | 5.8 | Serine/threonine-protein kinase WNK1 | Wnk1/Osr1 | _LQKSIS(ph)NPPGSNLR_ |
| 30 | 5.7 | Liprin-β-1 | Ppfibp1 | _RRPS(ph)DENSITPSEVQ |
Sites are ranked 1–30 based on fold response to the PDE inhibitor treatment. The underlined gene symbols in bold type face represent some of the phosphoproteins shown in Figs. 2–4. Each PDE inhibitor has an isozyme selectivity of at least 30-fold if used at an appropriate concentration. Data are from the 1-h time point (Golkowski et al., 2016).