Different approaches to targeted proteomics are used to study cAMP signaling
| Targeting Approach | Description | References |
|---|---|---|
| Affinity resin with chemically immobilized cAMP | cAMP is chemically immobilized onto agarose beads and used for affinity purification | Scholten et al., 2006; Kovanich et al., 2010; Aye et al., 2012; Burgers et al., 2012 |
| Affinity resin with chemically immobilized IBMX | The nonselective PDE inhibitor IBMX is chemically immobilized onto agarose beads and used for affinity purification | Corradini et al., 2015 |
| Protein tag–mediated immunoprecipitation | A protein of interest is tagged with a peptide or fluorescent protein and expressed in the target cells or tissues. The recombinant protein is purified from whole-cell lysates using antibodies against the peptide/protein tag | Means et al., 2011; Schmoker et al., 2018 |
| Enzyme-mediated proximity proteomics | A protein of interest is tagged with an engineered promiscuous biotin ligase and expressed in the target cells or tissues. All biotinylated proteins are purified from whole-cell lysates using streptavidin beads | Smith et al., 2018 |