TABLE 1

Competition-binding data of 125I-CYP (75 pM) and Sal with 10 µM Gpp(NH)p. Original binding curves are shown in Fig. 3, C and D

36°C38°C
pM%pM%
IC50fractionIC50fraction
Control

Ratio high-affinityto low-affinity state
High-affinity state3.4 ± 1.439 ± 25.6 ± 1.538 ± 2
Low-affinity state7,713 ± 65761 ± 29,838 ± 1,45062 ± 2
0.640.62
Gpp(NH)p

Ratio high-affinityto low-affinity state
High-affinity state13 ± 2.422 ± 36.3 ± 2.824 ± 3
Low-affinity state9,398 ± 58378 ± 312,830 ± 77576 ± 3
0.280.31