TABLE 3

Actions of JNJ-118 on rectification and conductance of GluA2 coexpressed with wild-type or mutated forms of γ8 and γ2

Summary data for measures of current rectification (Vb and RI), channel properties (γ and Po, peak), and kainate efficacy (IKA/IGlu) presented as mean ± S.D. from (n) patches. Also shown are unpaired or paired mean differences (upMD and pMD) with 95% confidence intervals (lower bound, upper bound) and P values from two-sample tests. Details as for Table 2.

TARP EffectDrug Effect
ControlupMD [95% CI]P value+JNJ-118pMD [95% CI]P value
Vb (mV)
 GluA2−60.8 ± 4.7 (6)−65.2 ± 8.0 (6)−4.37 [−8.65, −0.38]0.47
 GluA2/γ8−33.1 ± 5.5 (8)27.7 [22.7, 32.7]0.0087−41.1 ± 4.4 (8)−8.05 [−11.0, −6.34]0.039
 GluA2/γ8.dm−45.5 ± 3.4 (7)15.4 [11.0, 19.4]0.010−46.6 ± 3.6 (7)−1.11 [−4.51, 1.96]0.69
 GluA2/γ2−25.3 ± 9.7 (6)35.5 [24.7, 41.5]0.015−28.1 ± 8.3 (6)−2.79 [−5.38, 0.34]0.47
 GluA2/γ2.dm−28.1 ± 5.9 (8)32.8 [27.3, 37.7]0.0087−41.1 ± 6.8 (8)−13.0 [−15.3, −11.4]0.039
RI (I+60/I–60)
 GluA20.04 ± 0.03 (5)0.06 ± 0.04 (5)0.013 [−0.020, 0.070]1.00
 GluA2/γ80.37 ± 0.21 (8)0.33 [0.22, 0.51]0.0200.14 ± 0.08 (8)−0.24 [−0.35, −0.16]0.055
 GluA2/γ8.dm0.25 ± 0.10 (7)0.20 [0.14, 0.28]0.0280.24 ± 0.08 (7)−0.007 [−0.065, 0.053]1.00
 GluA2/γ20.44 ± 0.21 (6)0.40 [0.27, 0.60]0.0390.45 ± 0.25 (6)0.013 [−0.025, 0.071]1.00
 GluA2/γ2.dm0.32 ± 0.09 (8)0.27 [0.22, 0.34]0.0200.10 ± 0.05 (8)−0.21 [−0.27, −0.15]0.055
γ (pS)
 GluA217.3 ± 4.4 (9)17.9 ± 4.2 (9)0.64 [−1.71, 2.71]1.00
 GluA2/γ832.1 ± 7.6 (9)14.7 [9.18, 20.0]0.003520.6 ± 4.7 (9)−11.4 [−14.7, −8.3]0.028
 GluA2/γ8.dm27.9 ± 7.3 (8)10.6 [5.77, 17.0]0.009729.0 ± 7.4 (8)1.1 [−3.3, 6.9]1.00
 GluA2/γ228.5 ± 8.3 (10)11.2 [6.2, 17.9]0.009728.4 ± 4.3 (10)−0.051 [−2.95, 3.67]1.00
 GluA2/γ2.dm26.2 ± 9.7 (13)8.88 [3.32, 14.7]0.1018.1 ± 6.6 (13)−8.10 [−11.50, −5.49]0.0054
Po, peak
 GluA20.33 ± 0.22 (9)0.34 ± 0.11 (9)0.0050 [−0.092, 0.080]1.00
 GluA2/γ80.68 ± 0.12 (9)0.35 [0.19, 0.49]0.0110.52 ± 0.15 (9)−0.16 [−0.27, −0.043]0.14
 GluA2/γ8.dm0.59 ± 0.17 (8)0.25 [0.061, 0.41]0.190.57 ± 0.19 (8)−0.020 [−0.14, 0.15]1.00
 GluA2/γ20.77 ± 0.15 (10)0.44 [0.25, 0.58]0.00450.72 ± 0.16 (10)−0.05 [−0.090, 0.0017]0.32
 GluA2/γ2.dm0.76 ± 0.07 (13)0.43 [0.27, 0.55]<0.00010.52 ± 0.14 (13)−0.24 [−0.28, −0.18]0.0029
IKA/IGlu
 GluA20.006 ± 0.005 (7)0.005 ± 0.006 (7)−0.001 [−0.004,0.0002]1.00
 GluA2/γ80.49 ± 0.20 (10)0.48 [0.36, 0.60]0.000720.31 ± 0.19 (10)−0.18 [−0.21, −0.13]0.0059
 GluA2/γ2.dm0.50 ± 0.06 (10)0.50 [0.45, 0.53]0.000720.37 ± 0.08 (10)−0.13 [−0.15, −0.082]0.0078