Gene | Vehicle (n = 6) | ZINC13407541 (n = 6) | 8n (n = 7) | 13a (n = 5) | p-Value | |
---|---|---|---|---|---|---|
Osteoblast lineage | ||||||
Fgfr1 | 1.00 ± 0.35 | 0.51 ± 0.20** | 0.56 ± 0.23** | 0.21 ± 0.07*** | 0.0001 | |
Fgfr2 | 1.00 ± 0.12 | 0.98 ± 0.29 | 0.94 ± 0.32 | 0.44 ± 0.06** | 0.0010 | |
Fgfr3 | 1.00 ± 0.25 | 1.14 ± 0.24 | 1.16 ± 0.21 | 0.42 ± 0.17*** | <0.0001 | |
Fgfr4 | 1.00 ± 0.59 | 0.53 ± 0.25* | 0.45 ± 0.19** | 0.25 ± 0.06*** | 0.0057 | |
Fgf1 | 1.00 ± 0.25 | 1.14 ± 0.40 | 1.01 ± 0.39 | 0.38 ± 0.15* | 0.0019 | |
Fgf2 | 1.00 ± 0.32 | 1.04 ± 0.31 | 1.01 ± 0.27 | 0.52 ± 0.16* | 0.0081 | |
Fgf23 | 1.00 ± 0.33 | 0.88 ± 0.18 | 0.93 ± 0.41 | 0.46 ± 0.15* | 0.0189 | |
Dmp1 | 1.00 ± 0.48 | 0.77 ± 0.18 | 0.88 ± 0.16 | 0.38 ± 0.16*** | 0.0002 | |
Osteopontin | 1.00 ± 0.25 | 0.55 ± 0.11** | 0.56 ± 0.16** | 0.64 ± 0.12*** | <0.0001 | |
Bsp | 1.00 ± 0.34 | 0.87 ± 0.27 | 0.95 ± 0.29 | 0.90 ± 0.18 | 0.8607 | |
Mepe | 1.00 ± 0.16 | 1.68 ± 0.35* | 1.86 ± 0.44* | 5.42 ± 0.77*** | <0.0001 | |
Col1 | 1.00 ± 0.28 | 0.55 ± 0.16** | 0.62 ± 0.23* | 0.30 ± 0.10*** | <0.0001 | |
Alp | 1.00 ± 0.26 | 0.66 ± 0.20* | 0.60 ± 0.19** | 0.36 ± 0.08*** | 0.0001 | |
Osteocalcin | 1.00 ± 0.27 | 1.06 ± 0.32 | 1.25 ± 0.39 | 3.36 ± 0.97*** | <0.0001 | |
Runx2-II | 1.00 ± 0.23 | 0.82 ± 0.41 | 0.91 ± 0.31 | 0.21 ± 0.11*** | 0.0003 | |
Opg | 1.00 ± 0.20 | 1.02 ± 0.33 | 0.96 ± 0.29 | 0.92 ± 0.37 | 0.9488 | |
RankL | 1.00 ± 0.16 | 0.85 ± 0.18 | 0.97 ± 0.37 | 0.87 ± 0.11 | 0.6112 | |
Fzd2 | 1.00 ± 0.38 | 0.51 ± 0.21** | 0.62 ± 0.16* | 0.17 ± 0.08*** | <0.0001 | |
Wnt10b | 1.00 ± 0.27 | 0.53 ± 0.24** | 0.64 ± 0.16* | 0.12 ± 0.06*** | <0.0001 | |
Axin2 | 1.00 ± 0.25 | 0.57 ± 0.21** | 0.67 ± 0.17* | 0.11 ± 0.06*** | <0.0001 | |
Osteoclast | ||||||
Trap | 1.00 ± 0.17 | 0.91 ± 0.24 | 1.08 ± 0.27 | 0.86 ± 0.22 | 0.3383 | |
Mmp9 | 1.00 ± 0.21 | 0.81 ± 0.39 | 1.10 ± 0.34 | 1.11 ± 0.13 | 0.2358 | |
Chondrocyte | ||||||
Collagen II | 1.00 ± 0.45 | 3.72 ± 1.08* | 3.43 ± 2.27* | 14.4 ± 2.65*** | <0.0001 | |
VegfA | 1.00 ± 0.38 | 0.83 ± 0.51 | 0.77 ± 0.48 | 0.81 ± 0.27 | 0.7852 | |
Adipocyte | ||||||
PPARγ2 | 1.00 ± 0.32 | 0.43 ± 0.29** | 0.48 ± 0.31* | 0.10 ± 0.08*** | 0.0001 | |
aP2 | 1.00 ± 0.13 | 0.78 ± 0.15* | 0.70 ± 0.16** | 0.23 ± 0.11*** | <0.0001 | |
Lpl | 1.00 ± 0.34 | 0.47 ± 0.23** | 0.54 ± 0.24* | 0.17 ± 0.07*** | <0.0001 |
aP2, adipocyte fatty acid-binding protein 2; Bsp, bone sialoprotein; Col1, type I collagen; Fzd2, frizzled class receptor 2; Lpl, lipoprotein lipase; Mmp9, matrix metallopeptidase 9; Opg, osteoprotegerin; PPARg2, peroxisome proliferator-activated receptor gamma 2; RankL, receptor activator of nuclear factor kappa B ligand; Runx2-II, type II runt-related transcription factor-2; Trap, tartrate-resistant acid phosphatase; VegfA, vascular endothelial growth factor A.