TABLE 4

Gene-expression profiles in bone of Hyp mice treated with ZINC13407541 and its analogs

Data are expressed as the fold changes relative to the housekeeping gene β-actin subsequently normalized to control mice (n = 5–7). *, **, and *** indicate statistically significant difference from vehicle control group. P values were determined by one-way ANOVA with Tukey’s multiple-comparisons test.

GeneVehicle
(n = 6)
ZINC13407541
(n = 6)
8n
(n = 7)
13a
(n = 5)
p-Value
Osteoblast lineage
Fgfr11.00 ± 0.350.51 ± 0.20**0.56 ± 0.23**0.21 ± 0.07***0.0001
Fgfr21.00 ± 0.120.98 ± 0.290.94 ± 0.320.44 ± 0.06**0.0010
Fgfr31.00 ± 0.251.14 ± 0.241.16 ± 0.210.42 ± 0.17***<0.0001
Fgfr41.00 ± 0.590.53 ± 0.25*0.45 ± 0.19**0.25 ± 0.06***0.0057
Fgf11.00 ± 0.251.14 ± 0.401.01 ± 0.390.38 ± 0.15*0.0019
Fgf21.00 ± 0.321.04 ± 0.311.01 ± 0.270.52 ± 0.16*0.0081
Fgf231.00 ± 0.330.88 ± 0.180.93 ± 0.410.46 ± 0.15*0.0189
Dmp11.00 ± 0.480.77 ± 0.180.88 ± 0.160.38 ± 0.16***0.0002
Osteopontin1.00 ± 0.250.55 ± 0.11**0.56 ± 0.16**0.64 ± 0.12***<0.0001
Bsp1.00 ± 0.340.87 ± 0.270.95 ± 0.290.90 ± 0.180.8607
Mepe1.00 ± 0.161.68 ± 0.35*1.86 ± 0.44*5.42 ± 0.77***<0.0001
Col11.00 ± 0.280.55 ± 0.16**0.62 ± 0.23*0.30 ± 0.10***<0.0001
Alp1.00 ± 0.260.66 ± 0.20*0.60 ± 0.19**0.36 ± 0.08***0.0001
Osteocalcin1.00 ± 0.271.06 ± 0.321.25 ± 0.393.36 ± 0.97***<0.0001
Runx2-II1.00 ± 0.230.82 ± 0.410.91 ± 0.310.21 ± 0.11***0.0003
Opg1.00 ± 0.201.02 ± 0.330.96 ± 0.290.92 ± 0.370.9488
RankL1.00 ± 0.160.85 ± 0.180.97 ± 0.370.87 ± 0.110.6112
Fzd21.00 ± 0.380.51 ± 0.21**0.62 ± 0.16*0.17 ± 0.08***<0.0001
Wnt10b1.00 ± 0.270.53 ± 0.24**0.64 ± 0.16*0.12 ± 0.06***<0.0001
Axin21.00 ± 0.250.57 ± 0.21**0.67 ± 0.17*0.11 ± 0.06***<0.0001
Osteoclast
Trap1.00 ± 0.170.91 ± 0.241.08 ± 0.270.86 ± 0.220.3383
Mmp91.00 ± 0.210.81 ± 0.391.10 ± 0.341.11 ± 0.130.2358
Chondrocyte
Collagen II1.00 ± 0.453.72 ± 1.08*3.43 ± 2.27*14.4 ± 2.65***<0.0001
VegfA1.00 ± 0.380.83 ± 0.510.77 ± 0.480.81 ± 0.270.7852
Adipocyte
PPARγ21.00 ± 0.320.43 ± 0.29**0.48 ± 0.31*0.10 ± 0.08***0.0001
aP21.00 ± 0.130.78 ± 0.15*0.70 ± 0.16**0.23 ± 0.11***<0.0001
Lpl1.00 ± 0.340.47 ± 0.23**0.54 ± 0.24*0.17 ± 0.07***<0.0001
  • aP2, adipocyte fatty acid-binding protein 2; Bsp, bone sialoprotein; Col1, type I collagen; Fzd2, frizzled class receptor 2; Lpl, lipoprotein lipase; Mmp9, matrix metallopeptidase 9; Opg, osteoprotegerin; PPARg2, peroxisome proliferator-activated receptor gamma 2; RankL, receptor activator of nuclear factor kappa B ligand; Runx2-II, type II runt-related transcription factor-2; Trap, tartrate-resistant acid phosphatase; VegfA, vascular endothelial growth factor A.