TABLE 1

Primers used for 5′ RACE, 3′ RACE, and amplification of full-length Phh-grd and Phh-lcch3

Primers	Sequences (5′-3′)
Phh-grd 5′ RACE first PCR	CGAAATCGGGCCCATGCTTC
Phh-grd 5′ RACE nested PCR	CGGGCCCATGCTTCGTACC
Phh-grd 3′ RACE first PCR	GACTTTATCAAGATGGACGAGTTC
Phh-grd 3′ RACE nested PCR	GGACGAGTTCTTTATTCCAGC
Phh-grd full forward	ATTTTCCGTGGTGGAATTTTTTTTC
Phh-grd full Xho1 forward^a	CTGGCGGCCGCTCGAGATGGCGTCGATGTTCGGA
Phh-grd full reverse	GAATACATAGTAAAAATAAATAAAT
Phh-grd full Apa1 reverse^a	GAATACATAGTAAAAATAAATAAATGGGCCCGAGCTTGATCTGGT
Phh-lcch3 5′ RACE first PCR	CGTTGTCGTGAAACGCATACC
Phh-lcch3 5′ RACE nested PCR	TTTCAGCAAAATCACCCGCC
Phh-lcch3 full Xho1 forward^a	CTGGCGGCCGCTCGAGGGAATGATGATGCAATGCAGC
Phh-lcch3 full Apa1 reverse^a	GCAATGAGTAGTAAATATTTATACGGGGCCCGAGCTTGATCTGGT

^aPrimers Xho1 and Apa1 were designed with extensions (in italic) for recombination with the expression vector PTB207 linearized by Xho1 and Apa1.