Cloning of cDNAs Encoding G Protein-Coupled Receptor Expressed in Human Endothelial Cells Exposed to Fluid Shear Stress

doi:10.1006/bbrc.1997.7734

Biochemical and Biophysical Research Communications

Volume 240, Issue 3, 26 November 1997, Pages 737-741

https://doi.org/10.1006/bbrc.1997.7734 Get rights and content

Abstract

A cDNA library of human umbilical vein endothelial cells exposed to fluid shear stress was constructed to search for functional endothelial genes expressed under flow conditions, and cDNAs encoding members of the G protein-coupled receptor (GPCR) family were cloned by a polymerase chain reaction (PCR) method using degenerate oligonucleotide primers. One of the two GPCR clones obtained was edg-1, and the other clone is a novel gene named FEG-1 that encodes a 375-amino acid protein similar to the receptors for both angiotensin II and chemokines. Reverse transcriptase-PCR showed that the FEG-1 and edg-1 mRNA levels in endothelial cells increased markedly in response to fluid flow. This suggests that FEG-1 and edg-1 may be receptor genes that play important roles in the regulation of endothelial function under physiological blood flow conditions.

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¹: To whom correspondence should be addressed at Institute for Life Science Research, Asahi Chemical Industry Co., Ltd., 2-1, Samejima, Fuji-City, Shizuoka, 416 Japan. Fax: +81-545-62-3329. E-mail: [email protected].

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Regular ArticleCloning of cDNAs Encoding G Protein-Coupled Receptor Expressed in Human Endothelial Cells Exposed to Fluid Shear Stress

Abstract

Biochem. Biophys. Res. Commun.

FEBS Lett.

J. Biol. Chem.

J. Biol. Chem.

Biochem. Biophys. Res. Commun.

Biochem. Biophys. Res. Commun.

FEBS Lett.

Frontiers Med. Biol. Engng.

J. Clin. Invest.

Science

Regular Article
Cloning of cDNAs Encoding G Protein-Coupled Receptor Expressed in Human Endothelial Cells Exposed to Fluid Shear Stress