Regular Article
Differential Expression of Signal Transducers and Activators of Transcription during Human Adipogenesis

https://doi.org/10.1006/bbrc.2001.4460Get rights and content

Abstract

Signal Transducers and Activators of Transcription (STATs) display unique expression patterns upon induction of differentiation of murine 3T3-L1 preadipocytes into adipocytes. During differentiation, expression of STAT1 and STAT5 increase, while STAT3 and STAT6 remain relatively unchanged. Here, we determined whether human subcutaneous preadipocytes expressed STATs and if the pattern of expression changed during adipogenesis. We found by Western blot analysis that freshly isolated preadipocytes expressed STAT1, STAT3, STAT5, and STAT6, but not STAT2 and STAT4. Induction of preadipocyte differentiation with 1-methyl-3-isobutylxanthine, dexamethasone, insulin, and BRL49653 decreased expression of STAT1, and increased expression of STAT3 and STAT5. STAT6 expression did not change during adipogenesis. Changes in expression of CCAAT/enhancer binding protein β (C/EBPβ), C/EBPδ, C/EBPα, and peroxisome proliferator-activated receptor γ were similar to murine cell lines. These results suggest that unlike the traditional adipogenic transcription factors, unique differences exist in STAT expression patterns between murine and human adipose cells.

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      A number of transcription factors, including peroxisome proliferator-activated receptor gamma (PPARγ) and the CCAATT enhancer binding protein (C/EBP) family, are required for adipogenic differentiation [3,4]. Recently, signal transducer and activator of transcription 3 (STAT3) was demonstrated to play a key role in adipogenesis through PPAR-dependent signaling [5,6], highlighting the importance of regulation of PPAR-STAT3 signaling in the context of high-fat-associated human diseases. Statins were primarily developed as inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, and have been used clinically for the treatment of dyslipidemia [7].

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    To whom correspondence should be addressed at Department of Nutrition, University of North Carolina at Chapel Hill, CB# 7400 McGavran-Greenberg Hall, Chapel Hill, NC 27599. Fax: (919) 966-7216. E-mail: [email protected].

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