Regular Article
Isolation of a Family of Organic Anion Transporters from Human Liver and Kidney

https://doi.org/10.1006/bbrc.2001.4774Get rights and content

Abstract

Five distinct organic anion transporter cDNAs, hOAT1-5, were isolated from human liver and kidney. hOAT1, 2, and 3 are homologous to their respective rat orthologues OAT1-3, whereas hOAT4 and 5 are novel clones that have not been identified in other species. hOAT1- and hOAT3-transfected cells showed uptake of p-aminohippurate and fluorescein. Cells expressing hOAT2 showed uptake of p-aminohippurate, methotrexate, cAMP, and α-ketoglutarate. Northern blot analysis indicated differential tissue distribution for the transporter transcripts. These results indicate the existence of a family of organic anion transporting proteins in humans distinct from the oatp-like family of transporters.

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Cited by (174)

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    Citation Excerpt :

    OAT2/Oat2 was previously cloned from rat, human and mouse, and its isoforms range from 535 to 546 amino acids in size. Rat Oat2 shares 88 and 79% amino acid sequence identity with mouse Oat2 and human OAT2, respectively (Simonson et al., 1994; Sun et al., 2001; Kobayashi et al., 2002). Tissue expression profile showed the highest expression of human OAT2 mRNA in liver and kidneys, followed by pancreas, small intestine, lung, brain, spinal cord and heart.

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Abbreviations used: PAH, p-aminohippurate; BSP, sulfobromophthalein; AKG, α-ketoglutarate; TCA, taurocholic acid; MTX, methotrexate; DHEAS, dehydroepiandrosterone sulfate.

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