Regular ArticleGlutathioneS-Transferase-Catalyzed Conjugation of Bioactivated Aflatoxin B1in Rabbit Lung and Liver
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Changes in antioxidant enzymes and locomotor activity of yellow mealworm larvae fed the mycotoxin zearalenone supplemented diet
2023, Journal of Stored Products ResearchPhysiological and behavioral effects of the mycotoxin deoxynivalenol in Tenebrio molitor larvae
2019, Journal of Stored Products ResearchCitation Excerpt :While CAT is known to prevent peroxidation (Kouadio et al., 2005), upregulation of GST reduces lipid peroxides through a Se-independent pathway (Boeira et al., 2012; Yan et al., 2013). Glutathione-S-transferase has also been shown to catalyze the conjugation of mycotoxins (i.e. AFB1) with GSH, leading to excretion of the mycotoxin (Stewart et al., 1996; Guilford and Hope, 2014). The observed decrease in total protein content in our DON treated experimental groups could be due to inhibition of protein synthesis, a main toxic effect of trichothecenes including DON (Ueno, 1985; Rocha et al., 2005).
Pulmonary modulation of benzo[a]pyrene-induced hemato-and hepatotoxicity in broilers
2010, Poultry ScienceCitation Excerpt :This response is indicative of cellular oxidative stress because of the fact that the presence of lipid peroxidation products (Tables 7 and 8) apparently causes the induction of the activity of this enzyme that takes part in toxic compound removal. It has been observed that the presence of active oxygen species, as a primary response, induces GST gene expression (Zimniak et al., 1997) as similarly observed with other xenobiotics (Stewart et al., 1996). There is persuasive evidence to support the induction GST and protection against a wide spectrum of cytotoxic, mutagenic, and carcinogenic chemicals (Reed, 1990).
Protective effect of Aquilegia vulgaris L. on aflatoxin B<inf>1</inf>-induced hepatic damage in rats
2006, Environmental Toxicology and PharmacologyGlutathione-S-transferase activity toward aflatoxin epoxide in livers of mastomys and other rodents
2002, ToxiconCitation Excerpt :For the measurement of the microsomal activity converting AFB1 to the other free lipophilic metabolites, the microsomal fraction was pre-incubated for 5 min at 37 °C in the same manner but without calf thymus DNA. For the measurement of cytosolic GST activity, the cytosol fraction (1.0 mg of protein) was pre-incubated for 5 min at 37 °C in 1 ml of the phosphate buffer solution in the presence of hamster liver microsomes (1.0 mg of protein) for generation of AFB1-epoxide (Stewart et al., 1996). After the pre-incubation, the reaction was started by adding 5 μg of AFB1 or 5 μg of AFB1 containing 2 μCi of (3H)AFB1, both in 10 μl of dimethyl sulfoxide, and continued by incubation in the dark at 37 °C in a shaking water bath. (
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