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15-Hydroxy-eicosatetraenoic acid has minor anti-inflammatory properties in colitis

  • General Inflammation
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Abstract

This study was performed to determine whether 15-HETE, which is the major metabolite of arachidonic acid (AA) in inflamed and normal human colonic tissue, has pro- or anti-inflammatory properties. To investigate these effects, 15-HETE (100 μg/kg/day) was administered rectally to mice with colitis induced by dextran sodium sulphate (DSS). Colons were removed and examined macroscopically and histologically and specimens were incubated with [14C]-AA and stimulated with A23187. Exogenous eicosanoids were separated by HPLC; the endogenously formed mediators were measured by radioimmunoassay. DSS produced a marked diffuse inflammatory response in the colon, associated with a raised inflammation score (mean 7.6 vs. <0.5) and a significant increase in endogenously formed metabolites PGE2, LTB4 and 12-HETE. 15-HETE treatment resulted in a slight decrease in inflammation score (6.4 vs. 7.6) and a slight, but not significant, decrease in endogenous LTB4.

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Authors and Affiliations

  1. Dept. of Pharmacology, Erasmus University, P.O. Box 1738, 3000 DR, Rotterdam, The Netherlands

    A. P. M. van Dijk, D. M. McCafferty & F. J. Zijlstra

  2. Dept. of Internal Medicine II, Erasmus University, P.O. Box 1738, 3000 DR, Rotterdam, The Netherlands

    J. H. P. Wilson

Authors
  1. A. P. M. van Dijk
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  2. D. M. McCafferty
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  3. J. H. P. Wilson
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  4. F. J. Zijlstra
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van Dijk, A.P.M., McCafferty, D.M., Wilson, J.H.P. et al. 15-Hydroxy-eicosatetraenoic acid has minor anti-inflammatory properties in colitis. Agents and Actions 38 (Suppl 2), C120–C121 (1993). https://doi.org/10.1007/BF01991157

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  • DOI: https://doi.org/10.1007/BF01991157

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