Accumulation of short DNA fragments in hydroxyurea treated mouse L-cells

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Abstract

Treatment of L-cells with hydroxyurea markedly inhibits the incorporation of [3H]thymidine into DNA. The 3H incorporation that persists during hydroxyurea inhibition is largely into 7S DNA chains. The labelled fragments can be chased into higher MW DNA, suggesting that they are intermediates in the replication process. This interpretation concurs with that of earlier reports which describe a similar effect of hydroxyurea on the replication of viral DNA.

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