Elsevier

Brain Research

Volume 521, Issues 1–2, 25 June 1990, Pages 347-351
Brain Research

Inhibition of serotonin uptake into mouse brain synaptosomes by ionophores and ion-channel agents

https://doi.org/10.1016/0006-8993(90)91565-XGet rights and content

Abstract

[3H]Serotonin uptake into mouse cerebrocortical synaptosomes was decreased by the K+ ionophore valinomycin, the K+ and Na+ ionophore gramicidin, and the proton ionophore carbonylcyanidem-chlorophenylhydrazone. The Na+/H+ exchanger monensin reduced uptake at non-depolarizing concentrations. Uptake was also decreased by inhibition of the Na+,K+-ATPase with ouabain and by tetrodotoxin-sensitive activation of voltage-dependent sodium channels with veratridine, batrachotoxin and scorpion venom. In contrast, the Ca2+ channel agents BAY K8644 and nimodipine were ineffective. The effect of reducing the Na+ gradient depended upon whether the internal Na+ concentration was raised (i.e. by scorpion venom, monensim) or the external Na+ concentration was lowered (37 mM NaCl in the medium).

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