Separation of a1, a2 and a3 subunits of the GABAA-benzodiazepine receptor complex by immunoaffinity chromatography

doi:10.1016/0006-8993(91)91556-G

Brain Research

Volume 563, Issues 1–2, 1 November 1991, Pages 325-328

https://doi.org/10.1016/0006-8993(91)91556-G Get rights and content

Abstract

Polyclonal antibodies raised to synthetic amino acid sequences of the rat γ-aminobutyric acid (GABA_A) receptor α₁-, α₂- and α₃-subunits selectively recognized single proteins with apparent molecular weight 51 kDa (P₅₁), 53 kDa (P₅₃) and 59 kDa (P₅₉), respectively, in GABA_A receptor preparations affinity purified from the brains of 5–10-day-old rats. The antibodies were coupled to Affigel 10, and the resulting immunoaffinity columns were used to isolate these proteins from affinity purified GABA_A receptors.

References (15)

G. Drexler et al.
A rapid and simple method for efficient coating of microtiter plates using low amounts of antigen in the presence of detergent
J. Immunol. Meth.
(1986)
M.J. Duggan et al.
Biochemical evidence for the existence of γ-aminobutyrate_A receptor isooligomers
J. Biol. Chem.
(1990)
K. Fuchs et al.
Evidence for the existence of several different α- and ß-subunits of the GABA/benzodiazepine receptor complex from rat brain
Neurosci. Lett.
(1989)
K. Fuchs et al.
Identification of α₂- and α₃-subunits of the GABA_A-benzodiazepine receptor complex purified from the brains of young rats
FEBS Lett.
(1990)
N. Green et al.
Immunogenic structure of the influence virus hemagglutinin
Cell
(1982)
P.R. Schofield
The GABA_A receptor: molecular biology reveals a complex picture
Trends Pharmacol. Sci.
(1989)
F.A. Stephenson et al.
Identification of the α₃-subunit in the GABA_A receptor purified bovine brain
FEBS Lett.
(1989)

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^☆: This work was supported by the Fonds zur Förderung der Wissenschaftlicken Forschung in Österreich.

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Short communicationSeparation of a1, a2 and a3 subunits of the GABAA-benzodiazepine receptor complex by immunoaffinity chromatography☆

Abstract

J. Immunol. Meth.

J. Biol. Chem.

Neurosci. Lett.

FEBS Lett.

Cell

Trends Pharmacol. Sci.

FEBS Lett.

Short communication
Separation of a₁, a₂ and a₃ subunits of the GABA_A-benzodiazepine receptor complex by immunoaffinity chromatography☆