Cell
Volume 58, Issue 5, 8 September 1989, Pages 823-831
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Article
MyoD is a sequence-specific DNA binding protein requiring a region of myc homology to bind to the muscle creatine kinase enhancer

https://doi.org/10.1016/0092-8674(89)90935-5Get rights and content

Abstract

MyoD is a skeletal muscle-specific protein that is able to induce myogenesis in a wide variety of cell types. In this report, we show that MyoD is a DNA binding protein capable of specific interaction with two regions of the mouse muscle creatine kinase gene upstream enhancer, both of which are required for full muscle-specific enhancer activity. MyoD shares antigenicity and DNA binding specificity with MEF1, a myocyte-specific DNA binding factor. The contiguous basic and myc homology regions of MyoD that are necessary and sufficient for specific DNA interaction are the same regions of the protein required to convert 10T12 fibroblasts into muscle. These findings suggest that the biological activity of MyoD is mediated via its capacity for specific DNA interaction.

References (26)

  • D.J. Bergsma et al.

    Delimitation and characterization of cis-acting DNA sequences required for the regulated expression and transcriptional control of the chicken skeletal α-actin gene

    Mol. Cell. Biol.

    (1986)
  • L.M. Boxer et al.

    The sarcomeric actin CArG-binding factor is indistinguishable from the c-fos serum response factor

    Mol. Cell. Biol.

    (1989)
  • T. Braun et al.

    A novel human muscle factor related to but distinct from MyoD1 induces myogeneic conversion in 10T12 fibroblasts

    EMBO J.

    (1989)
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