Molecular cloning and expression of a receptor for human tumor necrosis factor

doi:10.1016/0092-8674(90)90816-W

Cell

Volume 61, Issue 2, 20 April 1990, Pages 361-370

https://doi.org/10.1016/0092-8674(90)90816-W Get rights and content

Abstract

A human tumor necrosis factor (TNF) binding protein from serum of cancer patients was purified to homogeneity and partially sequenced. Synthetic DNA probes based on amino acid sequence information were used to isolate cDNA clones encoding a receptor for TNF. The TNF receptor (TNF-R) is a 415 amino acid polypeptide with a single membrane-spanning region. The extracellular cysteine-rich domain of the TNF-R is homologous to the nerve growth factor receptor and the B cell activation protein Bp50. Human embryonic kidney cells transfected with a TNF-R expression vector specifically bind both ¹²⁵I-labeled and biotinylated TNF-α. Unlabeled TNF-α and TNF-β were equally effective at displacing the binding of labeled TNF-α to TNF-R expressing cells. Northern analysis indicates a single species of mRNA for the TNF-R in a variety of cell types. Therefore, the soluble TNF binding protein found in human serum is probably proteolytically derived from the TNF-R.

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Cell

Molecular cloning and expression of a receptor for human tumor necrosis factor

Abstract

J. Biol. Chem.

Meth. Enzymol.

J. Biol. Chem.

J. Biol. Chem.

J. Biol. Chem.

J. Biol. Chem.

J. Biol. Chem.

J. Biol. Chem.

Cell

J. Mol. Biol.

Cell

J. Biol. Chem.

J. Biol. Chem.

J. Biol. Chem.

J. Biol. Chem.

J. Biol. Chem.

Cell

J. Biol. Chem.

Characterization of receptors for human tumour necrosis factor and their regulation by γ-interferon

Nature

The history, properties, and biological effects of cachectin

Biochemistry

An endotoxin-induced serum factor that causes necrosis of tumors

A high molecular weight component of the human tumor necrosis factor receptor is associated with cytotoxicity

Computer-based characterization of epidermal growth factor precursor

Nature

Identification of TNF-LT blocking factor(s) in serum and ultrafiltrates of human cancer patients

Lymphokine Res.

Expression cloning of a receptor for human granulocyte-macrophage colony-stimulating factor

EMBO J.

Tumor necrosis factors: gene structure and biological activities

High efficiency gene transfer into mammalian cells

Characteristics of a human cell line transformed by DNA from human adenovirus type 5

J. Gen. Virol.

Interleukin-2 receptor β chain gene: generation of three receptor forms by cloned human α and β chain cDNA's

Science