Activation and depalmitoylation of Gsα

doi:10.1016/0092-8674(94)90445-6

Cell

Volume 77, Issue 7, 1 July 1994, Pages 1063-1070

https://doi.org/10.1016/0092-8674(94)90445-6 Get rights and content

Abstract

[³H]palmitate attached to mutationally activated α_s (α_s-R201C) turns over rapidly, compared with palmitate linked to normal α_s ( $t_{12} ≈ 2 min versus 90 min$ ); although α_s-R201C (unlike normal α_s) is predominantly found in the cytosol, [³H]palmitate is linked only to the membrane-bound pool of α_s, normal or mutant. Similarly, activation of wild-type α_s by isoproterenol, a β-adrenoceptor agonist that also induces membrane-to-cytosol translocation of α_s, dramatically accelerates depalmitoylation of α_s. Thus, activation-induced removal of palmitate provides an explanation for activation-induced shifts of α_s to the cytosol. Regulated palmitoylation cycles provide a potential general mechanism for controlling reversible changes in the cellular location and activity of a protein.

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Cell

ArticleActivation and depalmitoylation of Gsα

Abstract

Cell

FEBS Lett.

J. Biol. Chem.

J. Biol. Chem.

J. Biol. Chem.

J. Biol. Chem.

Cell

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Biochim. Biophys. Acta

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Biochem. Biophys. Res. Commun.

Cell

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J. Biol. Chem.

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Article
Activation and depalmitoylation of G_sα