Purification of membrane-bound aminopeptidase from rat brain: Identification of aminopeptidase M
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Cited by (13)
Recombinant expression and biochemical characterisation of two alanyl aminopeptidases of Trypanosoma congolense
2013, Experimental ParasitologyCitation Excerpt :Hence, the addition of metal chelators such as EDTA and 1,10-phenanthroline inhibited their activity (Table 3). It was also found that the antibiotic puromycin, which is used to distinguish between cytosolic alanyl APs and membrane bound APs (Gros et al., 1985), inhibited AP1 and AP2, implying that the two enzymes may indeed be cytosolic APs. The two APs were found to have similar inhibition constants for all the inhibitors tested.
Design, synthesis and biological evaluation of novel 1,2,3,4- tetrahydroisoquinoline-3-carboxylic acid derivatives as aminopeptidase N/CD13 inhibitors
2011, Bioorganic and Medicinal ChemistryCitation Excerpt :APN can preferentially release neutral or basic amino acids from the N-terminal of unsubstituted oligopeptides, amides and arylamide, with the exception of peptides with Pro in the penultimate position.3 APN is widely distributed in most of mammalian tissues such as kidney,4 intestine,5 liver,6 lung,7 brain,8 fibroblasts,9 vessels of smooth muscle,10 haematopoietic system,11 and so on. The widely distribution of APN associated with its broad substrate specificity suggests that APN has probably several physiological functions, such as protein modification, activation, and degradation as well as in the metabolism of biologically active peptides in tumor metastasis and leukemia.12,13
The Caenorhabditis elegans Orthologue of Mammalian Puromycin-sensitive Aminopeptidase Has Roles in Embryogenesis and Reproduction
2003, Journal of Biological ChemistryCitation Excerpt :Indeed, human PSA is completely inhibited by 100 μm puromycin (56), and IC50 values are reported to be of the order of 1 μm (57). In this respect, PAM-1 is more like aminopeptidase N (Ki ∼ 100 μm) (58). However, we cannot exclude the possibility that the N-terminal tag present on the recombinant nematode enzyme is influencing binding of puromycin, even though it appears not to affect interactions with the other inhibitors tested.
A specific enzyme assay for aminopeptidase M in rat brain
1992, Life Sciences