Cancer Letters

Cancer Letters

Volume 58, Issues 1–2, 14 June 1991, Pages 75-79
Cancer Letters

Quercetin-induced lipid peroxidation and DNa damage in isolated rat-liver nuclei

https://doi.org/10.1016/0304-3835(91)90026-EGet rights and content

Abstract

The extent of lipid peroxidation and DNA damage induced by quercetin were studied under aerobic conditions in isolated rat-liver nuclei. The effects of iron and copper ions on these two toxic oxidative processes were also investigated. Quercetin induced significant (P < 0.05) concentration-dependent nuclear lipid peroxidation concurrent with DNA degradation; these effects were enhanced by iron and copper ions. The results suggest that the reactive oxygen species generated by quercetin autoxidation, catalyzed by iron and copper ions, are responsible for the concurrent lipid peroxidation and DNA damage in isolated rat-liver nuclei.

References (35)

  • H.C. Birnboim

    DNA strand breaks in human leukocytes induced by superoxide anion

    Carcinogenesis

    (1986)
  • H.C. Birnboim

    A superoxide anion induced DNA strand-break metabolic pathway in human leukocytes

    Biochem. Cell Biol.

    (1988)
  • H.C. Birnboim et al.

    Fluorometric method for rapid detection of DNA strand breaks in human white blood cells produced by low doses of radiation

    Cancer Res.

    (1981)
  • R. Cox et al.

    A method for measuring DNA damage and repair in the liver in vitro

    Cancer Res.

    (1973)
  • Z. Dische

    Color reactions of nucleic acid components

  • E. Erturk et al.

    Hepatic tumors in Sprague-Dawley and Fisher-344 female rats exposed chronically to quercetin or its glycoside rutin

    Cancer Res.

    (1984)
  • W.W. Franke et al.

    The nuclear envelope and the architecture of the nuclear periphery

    J. Cell Biol.

    (1981)
  • Cited by (46)

    • A versatile flavonoid Quercetin: Study of its toxicity and differential gene expression in the liver of mice

      2022, Phytomedicine Plus
      Citation Excerpt :

      In our previous in vivo studies, the acute doses of apigenin and genistein (flavonoids) elevated the serum biomarkers level which was partially mediated through oxidative stress (Singh et al., 2012, 2014). Considering the reports (Robaszkiewicz et al., 2007; Sahu and Washington, 1991; Soria et al., 2010) that quercetin may behave as a prooxidant and can cause oxidative stress to the cells, certain important oxidative stress parameters were evaluated. LPO and GSH are two important parameters which indicate the state of the cell in oxidative stress condition.

    • Quercetin: Prooxidant Effect and Apoptosis in Cancer

      2018, Studies in Natural Products Chemistry
      Citation Excerpt :

      In this study, the most pronounced effect obtained using Ca2 + compared to Mg2 + was explained by the strong bonding of the latter causing stabilization of the reaction intermediates [52]. Sahu and coworkers demonstrated the degradation of DNA and lipid peroxidation by autoxidation caused by the incubation of hepatocyte nucleus from rats incubated with quercetin in the presence and absence of Cu2 +, and the latter caused a strong increase in the degradation process [53]. Oliveira and coworkers highlighted the mutagenic effect of quercetin in in vitro experiments and called attention to its autoxidation and consequent generation of ROS as a mechanism responsible for this biological effect [54].

    • Role of quercetin on Caco-2 cells against cytotoxic effects of alternariol and alternariol monomethyl ether

      2016, Food and Chemical Toxicology
      Citation Excerpt :

      However, there is also evidence of its prooxidative action (Durgo et al., 2007; Lombardi et al., 2012). Sahu and Washington (1991) and Soria et al. (2010) have found that the resultant prooxidant properties of Quer are responsible for its in vitro mutagenic and cytotoxic effects. Quer antioxidant and prooxidant effects largely relates to its dose in a given biological system.

    • Potential toxicity of quercetin: The repression of mitochondrial copy number via decreased POLG expression and excessive TFAM expression in irradiated murine bone marrow

      2014, Toxicology Reports
      Citation Excerpt :

      However, when quercetin exerts its antioxidant effect, it may be converted into potentially harmful oxidation products or subjected to in vitro oxidative degradation, resulting in the formation of an ortho-quinone and the subsequent production of ROS, such as superoxide and hydrogen peroxide (H2O2) [23]. The resultant prooxidant properties of quercetin are responsible for its in vitro mutagenic and cytotoxic effects [24,25]. Whether quercetin exerts antioxidant and prooxidant effects largely relates to its dose in a given biological system [26] and can be revealed by determining changes in the level of oxidative stress in the system when quercetin is added at different doses.

    View all citing articles on Scopus
    View full text