Elsevier

Neuroscience Letters

Volume 184, Issue 3, 30 January 1995, Pages 169-172
Neuroscience Letters

Fluoro Nissl Green: a novel fluorescent counterstain for neuroanatomy

https://doi.org/10.1016/0304-3940(94)11198-RGet rights and content

Abstract

Fluorescent neuroanatomic techniques, such as immunofluorescence and retrograde and anterograde tracing studies, derive great utility from their specificity. However, the specificity can be a drawback as well, in that it may be difficult to assess labeled neurons or neural processes in their cytoarchitectonic context. We report the characteristics of a newly synthesized fluorescent counterstain, Fluoro Nissl Green (3,8-diamino-10H-quindoline) with spectral characteristics similar to fluorescein. This Nissl-like counterstain can be used as a green neuronal counterstain for red-emitting markers such as rhodamine and Di-I.

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Present address: Department of Pathology and Neuropathology, New York University, Bellevue Medical Center, 27th Street and First Avenue, New York, NY 10016, USA.

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