Neuron
Volume 1, Issue 3, May 1988, Pages 241-248
Journal home page for Neuron

Article
cDNA clones coding for the structural subunit of a chicken brain nicotinic acetylcholine receptor

https://doi.org/10.1016/0896-6273(88)90145-6Get rights and content

Abstract

Nicotinic acetylcholine receptors (AChRs) immunoaffinity-purified from brains are composed of only two kinds of subunits rather than the four kinds present in muscle-type AChRs. Here we report the N-terminal protein sequences of the structural subunits of AChRs from rat and chicken brains and the cloning of full-length cDNAs for the chicken brain AChR structural subunit. Previously, the N-terminal amino acid sequence of the ACh-binding subunit of AChR immunoaffinity-purified from rat brain was shown to correspond to the cDNA α4. Thus, cDNA sequences are now known for both of the subunits that form one AChR subtype in vivo.

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