Cloning, localization, and permanent expression of a Drosophila octopamine receptor

doi:10.1016/0896-6273(90)90047-J

Neuron

Volume 4, Issue 3, March 1990, Pages 343-354

https://doi.org/10.1016/0896-6273(90)90047-J Get rights and content

Abstract

A cDNA for a member of the G protein-coupled receptor family was isolated from Drosophila using a probe derived from a human β₂-adrenergic receptor cDNA. This Drosophila receptor gene is localized at 99A10-B1 on the right arm of chromosome 3 and is preferentially expressed in Drosophila heads. The insect octopamine receptor has been permanently expressed in mammalian cells, where it mediates the attenuation of adenylate cyclase activity and exhibits a pharmacological profile consistent with an octopamine type 1 receptor. Sequence and pharmacological comparisons indicate that the octopamine receptor is unique but closely related to mammalian adrenergic receptors, perhaps as an evolutionary precursor.

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^†: Present address: Department of Pediatrics, The Kyoto Prefectural University of Medicine, Kawaramachi, Hirokoji, Kamikyoku, Kyoto, Japan 602.

^§: Present address: Section on Molecular Neurobiology, LPPS, NIAAA, ADAMHA, Rockville, Maryland 20852.

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Neuron

ArticleCloning, localization, and permanent expression of a Drosophila octopamine receptor

Abstract

Insect Biochem.

J. Biol. Chem.

FEBS Lett.

J. Biol. Chem.

Adv. Insect Physiol.

J. Biol. Chem.

Meth. Enzymol.

Brain Res.

FEBS Lett.

J. Mol. Biol.

Anal. Biochem.

FEBS Lett.

FEBS Lett.

Prog. Neurobiol.

Biochem. Pharmacol.

Comp. Biochem.

Octopamine

nature

Targets of octopamine action in the lobster: cyclic nucleotide changes and physiological effects in hemolymph, heart and exoskeletal muscle

J. Pharmacol. Exp. Ther.

Neurotransmitter synthesis in Limulus ventral nerve photoreceptors

Experientia

Transfer of proteins across membranes. II. Reconstitution of functional rough microsomes from heterologous components

J. Cell Biol.

Cloning and expression of a rat D2 dopamine receptor cDNA

Nature

Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease

Biochemistry

Molecular cloning and exoression of the cDNA for the hamster alpha1-adrenergic receptor

Effect of cyproheptadine on the octopamine-induced responses in the mammalian central nervous system

Experientia

A comprehensive set of sequence analysis programs of the VAX

Nucl. Acids Res.

Cloning of the gene and cDNA for mammalian β-adrenergic receptor and homology with rhodopsin

Nature

In situ hybridization to Drosophila salivary chromosomes with biotinylated DNA probes and alkaline phosphatase

Focus

Multiple receptor types for octopamine in the locust

J. Physiol.

Properties of modulatory octopamine receptors in the locust

CIBA Found. Symp.

Article
Cloning, localization, and permanent expression of a Drosophila octopamine receptor

Cloning and expression of a rat D₂ dopamine receptor cDNA

Molecular cloning and exoression of the cDNA for the hamster alpha₁-adrenergic receptor