Neuron
Volume 8, Issue 4, April 1992, Pages 775-785
Journal home page for Neuron

Article
The KA-2 subunit of excitatory amino acid receptors shows widespread expression in brain and forms ion channels with distantly related subunits

https://doi.org/10.1016/0896-6273(92)90098-XGet rights and content

Abstract

A new ionotropic glutamate receptor subunit termed KA-2, cloned from rat brain cDNA, exhibits high affinity for [3H]kainate (KD ≈ 15 nM). KA-2 mRNA is widely expressed in embryonic and adult brain. Homomeric KA-2 expression does not generate agonist-sensitive channels, but currents are observed when KA-2 is coexpressed with GluR5 or GIuR6 subunits. Specifically, coexpression of GluR5(R) and KA-2 produces channel activity, whereas homomeric expression of either subunit does not. Currents through heteromeric GluR5(Q)/KA-2 channels show more rapid desensitization and different current-voltage relations when compared with GIuR5(Q) currents. GluR6/KA-2 channels are gated by AMPA, which fails to gate homomeric GIuR6 receptor channels. These results suggest possible in vivo partnership relations for high affinity kainate receptors.

References (58)

  • S.G. Miller et al.

    Sequences of autophosphorylation sites in neuronal type II CaM kinase that control Ca2+-independent activity

    Neuron

    (1988)
  • D.T. Monaghan et al.

    The distribution of [3H]kainic acid binding sites in rat CNS as determined by autoradiography

    Brain Res.

    (1982)
  • H. Monyer et al.

    Glutamateoperated channels: developmentally early and mature forms arise by alternative splicing

    Neuron

    (1991)
  • K.B. Mullis et al.

    Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction

    Meth. Enzymol.

    (1987)
  • N. Nakanishi et al.

    A family of glutamate receptor genes: evidence for the formation of heteromultimeric receptors with distinct channel properties

    Neuron

    (1990)
  • D.K. Patneau et al.

    Kinetic analysis of interactions between kainate and AMPA: evidence for activation of a single receptor in mouse hippocampal neurons

    Neuron

    (1991)
  • R.M. Pruss et al.

    Agonist-activated cobalt identifies divalent cation-permeable kainate receptors on neurons and glial cells

    Neuron

    (1991)
  • J.H. Robinson et al.

    Kainic acid produces depolarisation of CA3 pyramidal cells in the in vitro hippocampal slice

    Brain Res.

    (1981)
  • K. Sakimura et al.

    Functional expression from cloned cDNAs of glutamate receptor species responsive to kainate and quisqualate

    FEBS Lett.

    (1990)
  • B. Sommer et al.

    RNA editing in brain controls a determinant of ion flow in glutamate-gated channels

    Cell

    (1991)
  • J.R. Unnerstall et al.

    Autoradiographic localization of high affinity [3H]kainic acid binding sites in the rat forebrain

    Eur. J. Pharmacol.

    (1983)
  • N. Unwin

    The structure of ion channels in membranes of excitable cells

    Neuron

    (1989)
  • R.J. Wenthold et al.

    Antibodies to a C-terminal peptide of the rat brain glutamate receptor subunit GluR-A, recognize a subpopulation of AMPA binding sites but not kainate sites

    FEBS Lett.

    (1990)
  • G.L. Westbrook et al.

    Cellular and synaptic basis of kainic acid induced epileptiform activity

    Brain Res.

    (1983)
  • C. Yanisch-Perron et al.

    Improved M13 phage cloning vectors and host strains: nucleotide sequences of M13 mp18 and pUC19 vectors

    Gene

    (1985)
  • A.B. Young et al.

    Excitatory amino acid receptors in the brain: membrane binding and receptor autoradiographic approaches

    Trends Pharmacol. Sci.

    (1990)
  • J. Boulter et al.

    Molecularcloning and functional expression of glutamate receptor subunit genes

    Science

    (1990)
  • C. Chen et al.

    High efficiency transformation of mammalian cells by plasmid DNA

    Mol. Cell. Biol.

    (1987)
  • Y.C. Cheng et al.

    Relationship between the inhibition constant (K) and the concentration of inhibitor which causes 50 percent inhibition (IC50) of an enzymatic reaction

    Biochem. Pharmacol.

    (1973)
  • Cited by (494)

    • A kainate receptor–selective RNA aptamer

      2020, Journal of Biological Chemistry
    View all citing articles on Scopus
    View full text