Use of a soluble tetrazolium compound to assay metabolic activation of intact β cells☆
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2012, Neuroscience ResearchCitation Excerpt :Cell viability was assessed 24 h after OGD using the MTS assay (Promega, Australia). The MTS assay measures the mitochondrial conversion of the tetrazolium salt to a water-soluble brown formazan salt, which is detected spectrophotometrically at 495 nm (Segu et al., 1998). MTS absorbance readings from controls and treatments were converted to percentage viability.
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2007, Free Radical Biology and MedicineValproic Acid, a Histone Deacetylase Inhibitor, Is an Antagonist for Oncolytic Adenoviral Gene Therapy
2006, Molecular TherapyCitation Excerpt :Both virus were amplified in their respective cell lines, purified using the AdenoPure adenovirus purification kit (PURESYN, Inc., Malvern, PA, USA), and titered using the Adeno-X Rapid Titer Kit (Clontech Laboratories, Inc., Mountain View, CA, USA). The metabolic viability of the cells was monitored using a MTS assay kit (CellTiter 96) from Promega (Madison, WI, USA) [20]. The assay is based on the older, widely used 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, which measures the colored formazan produced in a reduction reaction driven by nutrient metabolism, which can be quantified spectrophotometrically.
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Supported by grants from the Veterans Administration and the National Institutes of Health (DK 37312).